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polya buffer  (New England Biolabs)


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    Structured Review

    New England Biolabs polya buffer
    Plot showing the sequencing coverage (green), location of the 3’ ends of reads (orange) and splicing patterns (purple lines connecting splice donors and acceptors) for two Wiskott-Aldrich Syndrome vectors sequenced by Nanopore <t>direct</t> <t>RNA</t> technology (A) WAS LV5 sequencing data (contains a single A to T mutation in the potential cryptic <t>polyA</t> site). (B) Reference sequence of the Wiskott-Aldrich Syndrome vector, with various features annotated.
    Polya Buffer, supplied by New England Biolabs, used in various techniques. Bioz Stars score: 96/100, based on 1354 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/polya buffer/product/New England Biolabs
    Average 96 stars, based on 1354 article reviews
    polya buffer - by Bioz Stars, 2026-04
    96/100 stars

    Images

    1) Product Images from "An optimised protocol for quality control of gene therapy vectors using Nanopore direct RNA sequencing"

    Article Title: An optimised protocol for quality control of gene therapy vectors using Nanopore direct RNA sequencing

    Journal: bioRxiv

    doi: 10.1101/2023.12.03.569756

    Plot showing the sequencing coverage (green), location of the 3’ ends of reads (orange) and splicing patterns (purple lines connecting splice donors and acceptors) for two Wiskott-Aldrich Syndrome vectors sequenced by Nanopore direct RNA technology (A) WAS LV5 sequencing data (contains a single A to T mutation in the potential cryptic polyA site). (B) Reference sequence of the Wiskott-Aldrich Syndrome vector, with various features annotated.
    Figure Legend Snippet: Plot showing the sequencing coverage (green), location of the 3’ ends of reads (orange) and splicing patterns (purple lines connecting splice donors and acceptors) for two Wiskott-Aldrich Syndrome vectors sequenced by Nanopore direct RNA technology (A) WAS LV5 sequencing data (contains a single A to T mutation in the potential cryptic polyA site). (B) Reference sequence of the Wiskott-Aldrich Syndrome vector, with various features annotated.

    Techniques Used: Sequencing, Mutagenesis, Plasmid Preparation



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    Plot showing the sequencing coverage (green), location of the 3’ ends of reads (orange) and splicing patterns (purple lines connecting splice donors and acceptors) for two Wiskott-Aldrich Syndrome vectors sequenced by Nanopore <t>direct</t> <t>RNA</t> technology (A) WAS LV5 sequencing data (contains a single A to T mutation in the potential cryptic <t>polyA</t> site). (B) Reference sequence of the Wiskott-Aldrich Syndrome vector, with various features annotated.
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    Image Search Results


    Plot showing the sequencing coverage (green), location of the 3’ ends of reads (orange) and splicing patterns (purple lines connecting splice donors and acceptors) for two Wiskott-Aldrich Syndrome vectors sequenced by Nanopore direct RNA technology (A) WAS LV5 sequencing data (contains a single A to T mutation in the potential cryptic polyA site). (B) Reference sequence of the Wiskott-Aldrich Syndrome vector, with various features annotated.

    Journal: bioRxiv

    Article Title: An optimised protocol for quality control of gene therapy vectors using Nanopore direct RNA sequencing

    doi: 10.1101/2023.12.03.569756

    Figure Lengend Snippet: Plot showing the sequencing coverage (green), location of the 3’ ends of reads (orange) and splicing patterns (purple lines connecting splice donors and acceptors) for two Wiskott-Aldrich Syndrome vectors sequenced by Nanopore direct RNA technology (A) WAS LV5 sequencing data (contains a single A to T mutation in the potential cryptic polyA site). (B) Reference sequence of the Wiskott-Aldrich Syndrome vector, with various features annotated.

    Article Snippet: Artificial polyadenylation was carried out based on Crabtree et al. ( ) Reactions containing 15 μL of RNA (500 ng), 2 μL of polyA buffer, 2 μL of 10 mM ATP (NEB P0756S), 1 μL of E. coli Poly(A) Polymerase (NEB M0276S) and 0.5 μL of Murine RNase Inhibitor (NEB M0314S) were incubated for 30 minutes at 37°C, 20 minutes at 65°C and then 5 minutes at 98°C before being placed on ice.

    Techniques: Sequencing, Mutagenesis, Plasmid Preparation